Cloning and production of n. i bst / i nbi nicking endonuclease and related methods for the use of nicking endonucleases in the single strand displacement amplification -

摘要

The present invention relates to recombinant DNA which encodes a novel nicking endonuclease, N.BstNBI, and the production of N.BstNBI restriction endonuclease from the recombinant DNA utilizing PleI modification methylase. Related expression vectors, as well as the application of N.BstNBI and other nicking enzymes in non-modified strand displacement amplification, is disclosed also.