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method for cloning and expression of bsal restriktionsendonuklease and bsal methylase in e. coli

机译:bsal restriktionsendonuklease和bsal甲基化酶的克隆和表达方法大肠杆菌

摘要

The present invention relates to recombinant DNA which encodes the BsaI restriction endonuclease as well as BsaI methylase, expression of BsaI restriction endonuclease and BsaI methylase in E. coli cells containing the recombinant DNA, and purification of BsaI restriction endonuclease to near homogeneity. IMAGEThe present invention relates to recombinant DNA which encodes the BsaI restriction endonuclease as well as BsaI methylase, expression of BsaI restriction endonuclease and BsaI methylase in E. coli cells containing the recombinant DNA, and purification of BsaI restriction endonuclease to near homogeneity. IMAGE
机译:本发明涉及编码BsaI限制性核酸内切酶以及BsaI甲基化酶的重组DNA,在含有重组DNA的大肠杆菌细胞中表达BsaI限制性核酸内切酶和BsaI甲基化酶,以及将BsaI限制性核酸内切酶纯化至接近均一。图像技术领域本发明涉及编码BsaI限制性核酸内切酶以及BsaI甲基化酶的重组DNA,在包含重组DNA的大肠杆菌细胞中表达BsaI限制性核酸内切酶和BsaI甲基化酶,以及将BsaI限制性核酸内切酶纯化至接近均一。 <图像>

著录项

  • 公开/公告号DE60308934D1

    专利类型

  • 公开/公告日2006-11-23

    原文格式PDF

  • 申请/专利权人 NEW ENGLAND BIOLABS INC.;

    申请/专利号DE2003608934T

  • 发明设计人 ZHU ZHENYU;XU SHUANG-YONG;

    申请日2003-03-26

  • 分类号C12N15/09;C12N15/54;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/10;C12N9/16;C12N9/22;C12N15/55;C12N15/63;C12R1/07;

  • 国家 DE

  • 入库时间 2022-08-21 20:27:40

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