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A method and means for the determination of the relative heufigkeit of microorganisms in mixed populations
A method and means for the determination of the relative heufigkeit of microorganisms in mixed populations
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机译:测定混合种群中微生物相对遗传特性的方法和手段
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PCT No. PCT/NL96/00306 Sec. 371 Date Aug. 13, 1998 Sec. 102(e) Date Aug. 13, 1998 PCT Filed Jul. 29, 1996 PCT Pub. No. WO97/05282 PCT Pub. Date Feb. 13, 1997The present invention relates to a method for determining the relative abundance of individual species or lower phylogenetic subgroups of microorganisms in a mixed population of several microorganisms comprising the steps of: 1) providing a set of labeled in situ hybridization cluster oligonucleotide probes; 2) hybridization of said probes with a sample of the mixed population, and 3) quantitative analysis of the number of labeled microorganisms. Further it relates to a method for analyzing dynamics in relative abundance of individual microorganisms in a mixed population. Further it relates to a set of probes which are cluster specific and which are provided with at least one label for use in a method of the invention and to a kit of parts for determining the relative abundance of individual species of microorganisms in a mixed population of several microorganisms comprising such probes which together with suitable materials for pre-treating the sample and/or suitable materials for carrying out hybridization and/or suitable materials for analysis of the result of the hybridization.
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