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METHOD FOR CLASSIFYING GENOTYPE OF TYPE 2 PORCINE CIRCOVIRUS

机译:2型猪圆环病毒基因型分类的方法

摘要

PROBLEM TO BE SOLVED: To provide a method for accurately, quickly and easily classifying the genotype of PCV2.;SOLUTION: A genotype classification method for type 2 porcine circovirus gene includes the detection of the presence or absence of the cleavage site with one or more restriction enzymes selected from the group consisting of AvaII, BamHI, ClaI, AccI, NdeI and MboII in the gene of type 2 porcine circovirus; the above classification method includes the detection of the presence or absence of the above cleavage site in ORF2 of the type 2 porcine circovirus gene; and the genotype classification method performs the detection of the presence or absence of the cleavage site by PCR-RFLP method.;COPYRIGHT: (C)2008,JPO&INPIT
机译:解决的问题:提供一种准确,快速,轻松地对PCV2基因型进行分类的方法;解决方案:2型猪圆环病毒基因的基因型分类方法包括检测一个或多个裂解位点的存在与否。在2型猪圆环病毒的基因中的限制性酶选自AvaII,BamHI,ClaI,AccI,NdeI和MboII;上述分类方法包括检测2型猪圆环病毒基因ORF2中是否存在上述切割位点。基因型分类法通过PCR-RFLP方法检测切割位点的存在与否。;版权所有:(C)2008,日本特许厅&INPIT

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