NEW ANTIGEN DERIVED FROM HELICOBACTER PYLORI, ANTIGENIC COMPOSITION, AND DETECTION METHOD FOR HELICOBACTER PYLORI ANTIBODY

摘要

PPROBLEM TO BE SOLVED: To provide an antigenic composition containing the antigenic protein having high specificity to Helicobacter pylori antibody in the blood serum of Japanese or further of Asian. PSOLUTION: The antigenic composition including a part of CagA is a protein which is prepared by treating Helicobacter pylori with a buffer solution containing an alkylglycoside-based nonionic surfactant; feeding the solubilized extract to gel filtration chromatography; and concentrating and desalting the resultant polymer fraction. The protein is separated into three or more between 81 kDa and 125 kDa in the two-dimensional electrophoresis based on isoelectric point and molecular weight. PCOPYRIGHT: (C)2008,JPO&INPIT