Protein interaction detection system - proteins using fluorescent protein microdomains

摘要

Requires interacting polypeptides fused to drive the association of the fragments, the present invention further chromophores and fluorescent proteins do not alter the stability of the polypeptides which they stable and soluble and are fused to provide a protein-based, the system of interaction labeling and detection of proteins. In one embodiment, the fused (β chain 10, amino acids 198-214) to a fragment of 16 amino acids of GFP, the test protein X is operated so as not to change the solubility of the fusion protein. Fused (β chain 11, amino acids 215-230) to a fragment of 16 amino acids of GFP, the test protein Y and the second is operated so as not to change the solubility of the fusion protein. If X and Y interact, they are detected by complementation of the GFP third fragment consisting of (chain 1~9) GFP amino acids 1-198, and to approach the GFP chain.