Autologous hematopoietic stem cell preparation, method for producing the same, cryopreservation method, and use for treatment of traumatic diseases of the central nervous system

摘要

The invention applies to the field of cell transplantology and neurology and can be used to treat wound dystrophy of the central nervous system. The main idea of the invention is a preparation of haematopoietic stem cells (HSC), which is autologous HSC extracted from patient's peripheral blood enriched with cells containing CD34 antigen, in the final concentration of (40-100)x10SUP6/SUP cells/ml. Enriching the blood is performed using intermittent administration of the preparation of the granulocyte colony-stimulating factor (G-CSF) - for example, Neilogen in the glucose solution, according to a certain scheme. Also, the method of production, cryoconservation of the preparation and the method of treating traumatic diseases in the brain and spinal cord, using the new preparation. The developed system allows patients to be helped with neurological diseases efficiently and suffering minimum injuries.