A method of separating subpopulations animals according to genotype animals in each subgroup is a combination of multiple polymorphic or similar in the leptin gene and a single nucleotide polymorphism described above, the present invention, UASMS1 , UASMS2, UASMS3, EXON2-FB, provides a method and is selected from the group consisting of E2JW. Combination of single nucleotide polymorphisms above the leptin gene, the combination comprising alleles E2JW locus, also shows the increase of the softness of the meat, there may be one that shows the quality of the other traits of those animals in particular. Compared with the general population of that species of animal, feed intake of certain growth rate, body weight, carcass composition and carcass value, and the present invention, identifying an animal having a desirable phenotype on milk yield A method for, the method comprising determining also provides the presence of a combination of single nucleotide polymorphism or a single nucleotide polymorphism in the leptin gene of the animal.
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