METHOD OF FORMING STABLE COMPLEX OF TRANSCRIPTION PRODUCT OF DNA ENCODING ARBITRARY PEPTIDE WITH TRANSLATION PRODUCT, NUCLEIC ACID CONSTRUCT TO BE USED IN THE METHOD, COMPLEX FORMED BY THE METHOD, AND SCREENING OF FUNCTIONAL PROTEIN AND MRNA OR DNA ENCODING THE PROTEIN USING THE METHOD

摘要

PPROBLEM TO BE SOLVED: To provide a method for screening functional proteins from a group of amino acid random sequences without limiting diversity of the sequences. PSOLUTION: A method, by which a stable linkage between a genotype and a phenotype in a cell-free system is successfully achieved by using interaction between an RNA-binding protein and RNA, interaction between a DNA-binding protein and DNA, or a protein that inactivates a ribosome, is provided. Furthermore, a method for screening functional proteins by using the stable linkage is provided, and nucleic acid construct usable for the stable linkage is provided. PCOPYRIGHT: (C)2008,JPO&INPIT