Synthetic biomaterials incorporating bioactive factors through enzymatically degradable linkages

摘要

Synthetic biomaterials containing bioactive factors or modified bioactive factors that are covalently bound to the synthetic precursor components and/or biomaterials by an enzymatically degradable linkage are described herein. Further described are methods to covalently bind bioactive factors to synthetic biomaterials by means of enzymatic catalysis, the biomaterials produced therewith and the bioactive factors necessary for practicing these methods. The bioactive factors contain an amino acid sequence which can serve as a substrate domain for cross-linkable enzymes. The enzyme catalyzes the cross-linking reaction between the substrate domain of the bioactive factor and functional groups of the synthetic precursor components capable of forming the biomaterial and/or synthetic biomaterial susceptible to an enzymatically catalyzed cross-linking reaction. The biomaterials described herein may be used for localized delivery of the bioactive factors, for tissue repair and regeneration and in particular for regeneration of soft and hard tissue, such as skin, bone, tendons and cartilage.