The RGG gene which is connected functionally and the revelation system which includes the GTFG promoter

摘要

sutoreputokotsukasu gorudoni, other sutoreputokotsukasu stocks, and the production of the C repeat territory (CRR) by the other bacteria the revelation system which is based on the plasmid which can be used in order to reinforce is offered. As for this system, in order the synthesis of CRR from of the normal resident in characteristic to drive colon bacillus or shuttle plasmid pVA838 of sutoreputokotsukasu and the secretion, the promoter and secretion signal arrangement of gtfG which is combined with positive regulation gene rgg are utilized. This plasmid which is isolated from the colon bacillus was introduced by S. gorudoni easily, under reaching suitable condition, density of the protein which is secreted, almost 10 times increased in comparison with the production with surface protein revelation system (SPEX).