Method for removing malt fermented beverage remaining in membrane filter and method for rapid detection of microorganisms in fermented malt beverage

摘要

PPROBLEM TO BE SOLVED: To assay the viable count of a trace amount of bacterial cells mixed in a malt fermented beverage such as beer or a sparkling liquor without performing culture operation. PSOLUTION: The malt fermented beverage remaining on a membrane filter is washed and removed by removing bubbles on the membrane filter and then washing the membrane filter with a weakly alkaline aqueous solution when the malt fermented beverage is filtered through the membrane filter. The membrane filter subjected to the washing and removing treatment is further placed on an adenosine 5'-triphosphate (ATP)-free agar plate for about 1 h to reduce the background noise. The resultant membrane filter is placed in the ATP-free agar plate containing an ATP activator for microorganisms to activate the microorganisms and amplify the ATP in the cells. Thereby, the quantity of ATP emission is increased. As a result, the S/N ratio can remarkably be improved. PCOPYRIGHT: (C)2004,JPO