Cell culture evaluation system, cell culture evaluation method, and cell culture evaluation program

摘要

When culturing cells, estimate the lag time or minimum doubling time by using the average cell projected area of the cultured cell population or the rate of increase in the percentage of non-single cells as an evaluation parameter, and the cell population grows Provided are a cell culture evaluation system, a cell culture evaluation method, and a cell culture evaluation program that can objectively and accurately determine whether or not a stimulus has been stimulated. Obtain an image of the cell population to be statically cultured in the culture vessel, calculate the average projected area of the cells from the images for each culture time, and determine the lag time of the cell induction period from the calculated average projected area of the cells To do. In addition, single cells and non-single cells are discriminated from the image, the non-single cell increase rate is calculated from the non-single cell ratio of the cell population, and the minimum doubling time is calculated from the non-single cell increase rate. To be determined. In addition, it is determined whether the cell population is stimulated for growth from the ratio of non-single cells.