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Hybridization method and sensor chip hybridization and detection unit

机译:杂交方法及传感器芯片杂交检测单元

摘要

PPROBLEM TO BE SOLVED: To improve the hybridization efficiency by adopting a device so as to arrange and fix a nucleotide chain for sensing an elongated state. PSOLUTION: A hybridization sensing part 1a, etc., are composed so as to fix a reaction region R to be a site for hybridization between a nucleotide chain X for sensing and a target nucleotide chain Y having a base sequence complementary to the nucleotide chain X for sensing to an end of a scanning electrode C by dielectrophoretic actions while elongating the nucleotide chain X for sensing with an electric field. Thereby, a sensor chip equipped with the sensing part 1a, etc. and a method for hybridization using the hybridization sensing part and sensor chip are provided. PCOPYRIGHT: (C)2004,JPO
机译:

要解决的问题:通过采用一种装置来排列和固定用于感测伸长状态的核苷酸链来提高杂交效率。解决方案:杂交感测部分1a等被构成为将反应区域R固定为用于感测的核苷酸链X和具有与所述碱基互补的碱基序列的靶核苷酸链Y之间的杂交位点。用于通过介电泳作用感测到扫描电极C的末端的核苷酸链X,同时延长用于通过电场感测的核苷酸链X。从而,提供了配备有感测部1a等的传感器芯片以及使用杂交感测部和传感器芯片进行杂交的方法。

版权:(C)2004,日本特许厅

著录项

  • 公开/公告号JP4039201B2

    专利类型

  • 公开/公告日2008-01-30

    原文格式PDF

  • 申请/专利权人 ソニー株式会社;

    申请/专利号JP20020300924

  • 发明设计人 瀬川 雄司;眞峯 隆義;

    申请日2002-10-15

  • 分类号C12N15/09;C12M1/00;C12Q1/68;G01N27/00;G01N33/483;G01N37/00;

  • 国家 JP

  • 入库时间 2022-08-21 20:17:41

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