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The Tip acute angle the skin fretting chip one picking instrument tool which
The Tip acute angle the skin fretting chip one picking instrument tool which
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机译:Tip锐角皮肤微动芯片一种采摘工具
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摘要
A method for measuring the extent of skin aging which involves: the step of blunt-ending a fragment (TRF), which is obtained by treating a DNA extracted from an epidermal piece with a restriction enzyme and has a sticky end and a telomere-characteristic 3'-cohesive end, at both ends by terminal modification; the step of attaching linkers to both ends of the blunt-ended fragment followed by amplification by PCR; and the step of separating by electrophoresis the thus obtained amplification product which contains at least the whole telomere sequence in its part and detecting the average telomere length based on the distribution by using a labeled probe. By using this method, the extent of skin aging can be quickly and accurately measured with the use of a small amount of DNA.
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