Method of calculating the dissociation constant of the surface plasmon resonance analysis

摘要

(Noise width of the reference chip) the noise level is low and baseline wander object of the present invention, (the calculation of the dissociation constant of a molecule that interacts with the analyzed molecules immobilized on the metal surface in the surface plasmon resonance It is to realize the analysis method of signal change) of the reference chip is small, it is possible to obtain a measurement result with high reliability. In accordance with the present invention, and an optical detection means for detecting the state of surface plasmon resonance to measure a flow channel system comprising a cell formed on the metal film, the intensity of the light beam totally reflected at the metal film surface In the method of measuring the signal changes in the surface plasmon resonance in a state where using a surface plasmon resonance measurement device comprising Te, after replacing the liquid in the flow channel system, stopping the flow of the liquid, from the measurement result of the signal change, and metal How to calculate the dissociation constant of a molecule that interacts with the analyzed molecules immobilized on the surface is provided.