Chitin oligosaccharide elicitor-binding protein

摘要

The present inventors have isolated and purified elicitor binding protein in good yield by combining the development of the column with APEA derivative, precolumn for non-specific adsorption material removal, the elution method effective. Were successfully isolated a cDNA N-terminal thus obtained, and by using the internal chain amino acid sequence encoding the protein of the present invention from the rice cDNA library. In addition, when the purified anti-Con A-CEBiP antibody to examine the effect on the elicitor responsiveness of active oxygen production, active oxygen production was inhibited by pretreatment with the antibody, the protein chitin oligosaccharide elicitor response It is a receptor protein involved was suggested. Since induce blast resistance to rice, the protein of the present invention, the elicitor can be applied to the development of disease control novel techniques.