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KpnI Restriction Endonucleases with Reduced Star Activity
KpnI Restriction Endonucleases with Reduced Star Activity
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机译:KpnI限制性内切核酸酶的星活动减少
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摘要
Methods are provided for making restriction endonucleases with reduced star activity by one or more targeted mutations to a catalytic site within the restriction endonuclease. Examples of modifications to restriction endonucleases with significant sequence identity with KpnI are provided and reduced star activity demonstrated.
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