Antibodies Against an Extracellular Matrix Complex and Their Use in the Detection of Cancer

摘要

A method for assaying for cancer in a mammal. The method comprises attaching a trapping antibody to a surface, wherein the trapping antibody recognizes extracellular matrix (ECM) complexes. A biological fluid sample is contacted with the trapping antibody attached to the surface to thereby bind any ECM complex present in the biological fluid to the trapping antibody to form bound ECM complex. A signaling antibody is prepared and bound to bound ECM complex, wherein the signaling antibody recognizes ECM complex. The amount of signaling antibody bound to the surface is then quantitated, wherein accumulation of signaling antibody on the surface, above a background accumulation, indicates a positive result for cancer.