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Method of creating a library of bacterial clones with varying levels of gene expression
Method of creating a library of bacterial clones with varying levels of gene expression
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机译:创建具有不同基因表达水平的细菌克隆文库的方法
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摘要
The present invention relates to a method of creating DNA libraries that include an artificial promoter library and/or a modified ribosome binding site library and transforming bacterial host cells with the library to obtain a population of bacterial clones having a range of expression levels for a chromosomal gene of interest.
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