Hyperspectral fluorescence and absorption bioimaging

摘要

A system and method of hyperspectral chemical imaging (fluorescence or absorption based) to provide an automated approach for a more detailed analysis of disease status of a biological sample. When a biological sample is labeled with a fluorescent or light-absorbing contrast-enhancing agent, interactions between the contrast-enhancing agent and one or more constituents (or cellular components) of the biological sample may be manifested through spectral contents of a plurality of regions in a hyperspectral chemical image of the sample. Observations of such manifestations through analysis of corresponding spectral contents may greatly assist a user (e.g., a pathologist) in detecting and differentiating diseased portions of the stained sample. Hyperspectral chemical imaging may allow to identify multiple cellular components within a biological sample and to image their distribution within the sample, thereby assisting a pathologist to successfully and more accurately identify diseased portion(s) of the sample for further diagnosis and treatment.