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Methods For Making And Using Mass Tag Standards For Quantitative Proteomics
Methods For Making And Using Mass Tag Standards For Quantitative Proteomics
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机译:制备和使用定量蛋白质组学质量标签标准品的方法
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摘要
Disclosed are methods for producing peptide standards for quantitative proteomics. In one disclosed embodiment, chimeric polypeptides that are a combination of mass tags for multiple proteins are expressed in a host cell that is grown on an isotopically-altered medium. The mass tags in the chimeric polypeptide are separated by specific cleavage sites (such as trypsin cleavage sites), and upon treatment with an appropriate protein cleavage agent (such as trypsin) the constituent peptide standards are released. Methods of mass spectrometric analysis that employ the disclosed chimeric polypeptides (or the peptide standards liberated therefrom) also are disclosed.
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