Methods for matrix cleanup and analysis of drugs and metabolites in biological matrices

摘要

The invention is directed to the use of weak anion exchange (WAX) materials for trapping of negative and zwitterionic interfaces from biological matrices, and then reduction of the biological matrix effect in the quantitative analysis process of basic and neutral compounds present in the matrix. The sample preparation process includes adding the WAX cleanup step before or after or during the conventional extraction procedures like liquid-liquid extraction, protein precipitation, solid phase extraction and others. Such a step greatly enhances the selectivity of the extraction process via the removal of the majority of the contaminants and reduces the matrix effect in the quantitative analysis. In addition, the WAX-enhanced extraction is very simple versatile, rug and easy to be operated.