Methods for generating genetic diversity by Mutagenesis with permutations

摘要

Methods for generating genetic diversity in a sequence of polynucleotides or polypeptides to alter or improve the function of polynucleotide and polypeptide.Claim 1: a method to generate a polynucleotide that encodes a polypeptide that has a desired feature is characterised in that it comprises: (a) to align a Plurality of polypeptides that have regions of Sequence Homology to identify one or more regions of heterogene For Sequence; (b) to generate a consensus translation to at least a First region heterogeneity dE Sequence; (c) to generate a population of polynucleotides, where the population a population of polynucleotides encoding polypeptidesWhere the sequence corresponding to at least a First region of Sequence heterogeneity in the population of polypeptides consists of translation consensus generated in STEP (b); (d) linking the population of polynucleotides in a construction of expression vector; (e) to express the Construction generated in STEP (d) to provide in a host Cell expression of Pol ProductsIpu00e9ptidos; and (f) to evaluate the desired feature. The Method of claim 7, clause 4, wherein the Enzyme is EPSP synthase.