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Methods for generating genetic diversity by Mutagenesis with permutations

机译:通过置换诱变产生遗传多样性的方法

摘要

Methods for generating genetic diversity in a sequence of polynucleotides or polypeptides to alter or improve the function of polynucleotide and polypeptide.Claim 1: a method to generate a polynucleotide that encodes a polypeptide that has a desired feature is characterised in that it comprises: (a) to align a Plurality of polypeptides that have regions of Sequence Homology to identify one or more regions of heterogene For Sequence; (b) to generate a consensus translation to at least a First region heterogeneity dE Sequence; (c) to generate a population of polynucleotides, where the population a population of polynucleotides encoding polypeptidesWhere the sequence corresponding to at least a First region of Sequence heterogeneity in the population of polypeptides consists of translation consensus generated in STEP (b); (d) linking the population of polynucleotides in a construction of expression vector; (e) to express the Construction generated in STEP (d) to provide in a host Cell expression of Pol ProductsIpu00e9ptidos; and (f) to evaluate the desired feature. The Method of claim 7, clause 4, wherein the Enzyme is EPSP synthase.
机译:在多核苷酸或多肽的序列中产生遗传多样性以改变或改善多核苷酸和多肽的功能的方法。权利要求1:一种产生编码具有所需特征的多肽的多核苷酸的方法,其特征在于包括:(a )排列多个具有序列同源性区域的多肽,以鉴定一个或多个异源序列区域; (b)产生对至少第一区域异质性dE序列的共有翻译; (c)产生多核苷酸群体,其中该群体是编码多肽的多核苷酸群体,其中对应于该多肽群体中至少一个序列异质性的第一区域的序列由步骤(b)中产生的翻译共有序列组成; (d)在表达载体的构建中连接多核苷酸群体; (e)表达在步骤(d)中产生的构建体,以在宿主细胞中提供Pol ProductsIp u00e9ptidos; (f)评估所需功能。 8.根据权利要求7所述的方法,其中,所述酶是EPSP合酶。

著录项

  • 公开/公告号AR061365A1

    专利类型

  • 公开/公告日2008-08-20

    原文格式PDF

  • 申请/专利权人 ATHENIX CORPORATION;

    申请/专利号AR2007P102590

  • 发明设计人

    申请日2007-06-13

  • 分类号C12Q1/68;C12N15/10;C12N15/63;

  • 国家 AR

  • 入库时间 2022-08-21 20:08:53

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