Molecular typing of fungi of the complex Cryptococcus neoformans through the technique of PCR - RFLP

摘要

Molecular typing of fungi of the complex Cryptococcus neoformans through the technique of PCR - RFLP is a methodology for molecular typing of the fungus Cryptococcus neoformans in which a DNA fragment of 597pb gene is amplified by PCR and CAP59 cleaved with a mixture of two endonuclease S of restriction (commercial enzymes) for 3 hours at a temperature of 37 198 C and fragments are separated pOr 1.5% agarose gel electrophoresis, stained by etidio bromide and visualized in multiple ultraviolet light (UV) and / or registered in the system of fotodocumentau00e7u00e3o; and the cleavage of this fragment of 597 BP by restriction enzymes indicad, s allows to represent the samples according C Om serotypes defined by the kit cryptocheck