Process for preparing controlled layers of fibrin on solid surfaces

摘要

A method of preparing regulated fibrin layers on solid cell growth surfaces by adsorbing fibrinogen, preferably 2 µg / ml to 200 µg / ml, to the solid surface by adsorption from a fibrinogen solution in a first step, the treated surface is treated with a thrombin solution, preferably 0.1 U / ml to 10 U / ml, in a second step, which in the third step is treated with a fibrinogen-containing solution, preferably 200 µg / ml or higher and thrombin inhibitors that only inhibit thrombin in solution and surface-bound nicolithrombin, or heparinized blood plasma, while the thrombin solution phase and subsequent action with a fibrinogen-containing solution and thrombin inhibitors can be repeated. As a fibrinogen solution in the first step, anticoagulated blood plasma can be used. As the fibrinogen solution in the third step, heparinized blood plasma can be used. Antithrombin or antithrombin with heparin may be used as the thrombin inhibitor.