A NOVEL PROTEASE FOR INDUSTRIAL APPLICATIONS

摘要

A NOVEL PROTEASE FOR INDUSTRIAL APPLICATIONS The present invention relates to an extracellular enzyme protease obtained by growing the culture of Pseudomonas aeruginosa MCM B-327 isolated from vermiculture pit soil and deposited in MTCC, IMTECH, Chandigarh with designation MTCC 5270, in production medium of pH 7.0; containing soybean meal and tryptone as raw materials, at 30°C for 72 h. The organism was also able to produce protease using different agricultural products/ byproducts as protein sources. The partially purified non-collagenolytic, calcium independent protease with molecular weight 60 kDa has activity in pH range of 6.0 - 11.0 and temperature range of 25-65°C; stability in pH range of 6.0-10.0 and temperature 25-45°C. The protease activity was retained for 8 months when stored at ambient temperature. Ammonium sulphate precipitated enzyme was able to completely dehair animal skins and hides without chemicals like lime, sodium sulphide and calcium.