A RECOMBINANT ANTIBODY OR A SINGLE CHAIN FV FRAGMENT FOR RECOGNIZING EPITOPES EXPRESSED IN IOR C2 ANTIGEN

摘要

The invention relates to the obtention of novel recombinant antibodies from murine antibody IOR C5 produced by the hybridoma deposited with the ECCC 97061101 according to the Budapest Treaty. Said recombinant antibodies were obtained using recombinant DNA technology and are characterized in that they recognize antigen ior C2. The recombinant antibodies are specifically chimeric antibody, humanized antibody, and single chain Fv fragment. The chimeric antibody contains the variable domains of the murine immunoglobuiine and the constant regions of the human immunoglobuiine and has been specifically modified in the murine frameworks regions (FRs) and within the latter, in those areas that may result in an antigenic site for cells T. the Fv fragment contains the variable domains of murine immunoglobuiine. The invention also relates to the utilization of recombinant antibodies derived from murine antibody ior C5 in the diagnosis and therapy of colorectal tumors, the metastasis thereof and recurrences.