Kit and method for detection and identification of yeasts of clinical interest, through a amplificau00c7u00c3o isotu00c9rmica DNA followed by hibridau00c7u00c3o with specific oligonucleu00d3tidos and their uses.

摘要

This invenu00c7u00c3o relates to a process for the detection and identification of yeasts from clinical interest in the use of a technical amplificau00c7u00c3o isotu00c9rmica DNA using primers specific for oligonucleu00d3tidos not amplify a region of DNA rib Ossu00d3mico (e.g. the rDNA 265), followed by reverse hibridau00c7u00c3o products marked (e.g.With digoxigenina) with a panel of oligonucleu00d3tidos probes (e.g., immobilized on a support such as a nylon membrane solid) specific to each species. The occur of hibridau00c7u00c3o is subsequently detected by a method colorimu00c9trico.This invenu00c7u00c3o relates also to the development of a kit for the detection and identification of yeasts with clinical relevance based on the said process.In this way, the present invention is useful in the detection and identification of yeasts related to infections with the advantage of revealing a method simple, fast, reliable and low cost, and can be applied in the clinical area, pharmaceutical, veterinary, food and biotecnolu00d3gica.