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METHOD FOR REVEAL OF ONCOGENES RET/PTC IN PAPILLARY CARCINOMAS OF THYROID GLAND

机译:甲状腺乳头状癌中癌基因RET / PTC的暴露方法

摘要

Method for reveal of oncogenes RET/ in papillary carcinomas of thyroid gland includes extraction of RNA from tumor tissue with performing reaction of back transcription and amplification of transcripts with respective primers. Amplification is performed by means of quantitative polymerization of chain reaction with determination of levels of expression of thyhrozincynase and extracell domains of gene RET with following evaluation of ratio of those. If the ratio is larger than two, one states presence of oncogenes RET/PTC.
机译:用于揭示甲状腺乳头状癌中致癌基因RET /的方法包括从肿瘤组织中提取RNA,并进行反转录反应并用相应的引物扩增转录本。通过链式反应的定量聚合进行扩增,并测定酪氨酸锌合酶和RET基因的胞外域的表达水平,然后评估它们的比例。如果比率大于2,则表示存在癌基因RET / PTC。

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