Cultivating tendon cells made of non-embryonic pluripotent cells of mesenchymal origin, comprises cultivating the isolated cells in a culture medium under standard culture conditions in a culture container

摘要

Cultivating tendon cells made of non-embryonic pluripotent cells of mesenchymal origin, comprises cultivating the isolated cells in a culture medium under standard culture conditions in a culture container, where the cells before its complete confluence are further cultivated with an ascorbic acid and/or ascorbic acid-2-phosphate in a concentration of 25-75 mu g/ml transferred culture medium on the one hand and on the other hand a hyperosmolar treatment is subjected in a culture medium, whose osmolarity is adjusted to 350-500 mosmol/l.