CONDENSED PLASMID-LIPOSOMA COMPLEX FOR TRANSFECTION.

摘要

Composition of plasmid-liposome complexes for use in the transfection of a host cell with a gene contained in a plasmid comprising condensed plasmid molecules, said molecules being condensed with a polycationic condensing agent and suspended in an aqueous medium containing a non-electrolyte solute, said medium having an ionic strength lower than the ionic strength of a 10 mM solution of a monovalent ionizable salt, and cationic liposomes comprising a cationic vesicle-forming vesicle lipid, dimethyldioctadecylammonium bromide (DDAB), N- [1- (2-3-ditetradecyloxy) propyl] -N, N-dimethyl-N-hydroxyethylammonium (DMRIE) bromide, N- [1- (2, 3-dioleyloxy) propyl-N, N- dimethyl-N-hydroxyethylammonium (DORIE), or N- [1- (2,3-dioleyloxy) propyl] -N, N, N-trimethylammonium chloride (DOTMA), in which said complexes have a proportion of liposome lipid a plasmid greater than 5 nmoles of li I ask for liposome / µg of plasmid and less than 25 nmoles of liposome lipid / µg of plasmid and have a homogeneous size of less than 200 nm.