Ecrutinio for new inhibitors of protein based on a cell line genetically engineered containing an inducible gene overactivity and a compensatory gene.

摘要

A method for identifying the presence of an inhibitor of a target protein in a sample, comprising the steps of: a) contacting said sample with a cell, wherein said cell contains (i) a mutation of lethal overactivity inducible a gene affecting the target protein, wherein the activity of the target protein is essential to the cell; and (ii) a mutation in a second gene, wherein the mutation in the second gene functionally compensates for any reduction in the activity of the target protein; b) inducing the lethal overactivity mutation; and then c) determining the inhibition of protein by comparing the degree of survival of the cell in the presence and absence of said sample.