rational selection of putative nucleotide sequences from peptides or peptide identified.

摘要

Method of identifying precursors of a peptide comprising an amidated C-terminus, comprising the following steps: - Extraction of automated sequences from a public source; - Analysis of the files and import data OracleTM - Selection of sequences from a subset or from the entire database; - Search for precursors of a peptide comprising a C-terminal amidated using the sequence Y1-Y2-Y3-Y4-Y5 in which Y1 is a nucleotide sequence of 1 to 12 nucleotides or Y1 is suppressed, Y2 is a codon for Gly, Y3 and Y4 they are independently codons for Arg or Lys and Y5 is a nucleotide sequence of 1 to 21 nucleotides or Y5 is suppressed; - Verification of the absence of any codon AUG codon DETENTION between indicating the start of the reading frame and the rest sought and identification of such sequences; - Check; if found, the rest is an open frame type secuenciaconsensus Kozak reading; - Comparison of the average of the rest of the site with required by searching secondary structures required around of a maturation site such as the proximity of alpha-helices or beta-sheets; - Verification of the presence of a similarity of the sequences and other known sequences. - 25 -