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GROWING MEDIA FOR CHO CELLS AND ADAPTED CHO CELLS.
GROWING MEDIA FOR CHO CELLS AND ADAPTED CHO CELLS.
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机译:CHO细胞和自适应CHO细胞的生长介质。
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摘要
A biochemically defined culture medium for the cultivation of DHFR-genetically engineered CHO cells to produce a product selected from the group consisting of: human antibodies, humanized antibodies, CDR-grafted antibodies, tissue-type plasminogen activator, whose medium is free of protein, lipid and carbohydrate isolated from an animal source, wherein said medium comprises: (a) water; (b) an osmolality regulator to maintain the osmolality of the medium within the range of 200-350 mOsm; (c) a buffer to maintain the pH of the medium within the range of 6.5 to 7.5; (d) a monosaccharide energy source in the range of 1000-10,000 mg / liter; (e) amino acids, including L-glutamine in the range of 400-600 mg / ml; (f) a source of recombinant iron or a source of inorganic iron within the range of 0.25 to 5 mg / liter; (g) recombinant or synthetic insulin; (h) a cell development enhancer that is involved in the folate pathway selected from the group consisting of folic acid, vitamin B6, vitamin B12; (i) a lipid factor in the range of 0.05-10 mg / liter; wherein said medium is essentially free of hypoxanthine and / or thymidine.
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