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OLIGONUCLEOTIDES DIRECTED AT THE 23S RIBOSOMAL RNA GENE OF ACIDOBACTERIA AND USE THEREOF

机译:嗜酸细菌23S核糖体RNA基因中的寡核苷酸及其用途

摘要

The invention relates to oligonucleotides which are directed at the 23S ribosomal RNA gene of acidobacteria and to the use thereof. The rapid detection of bacteria is essential for determining the role of same in different processes, both of beneficial and harmful organisms. The purpose of the invention is to develop characteristic sequences that can be used to identify acidobacteria and the subgroups to which they belong in a quick and effective manner. Said bacterial division was identified based on previously-unknown sequences of the 23S ribosomal RNA gene of acidobacteria. Said oligonucleotides can be used to prepare primers for use in DNA amplification reactions as nucleic acid probes or for use in DNA/RNA arrays. The detection of acidobacteria and the differentiation of the subgroups thereof can be used with samples of different types, but preferably with environmental, biological and/or medical samples.
机译:本发明涉及针对酸性细菌的23S核糖体RNA基因的寡核苷酸及其用途。快速检测细菌对于确定细菌在有益和有害生物的不同过程中的作用至关重要。本发明的目的是开发可用于以快速有效的方式鉴定酸性细菌及其所属的亚组的特征序列。基于酸性细菌的23S核糖体RNA基因的先前未知序列鉴定所述细菌分裂。所述寡核苷酸可用于制备引物,所述引物用于DNA扩增反应中作为核酸探针或用于DNA / RNA阵列。酸性细菌的检测及其亚组的分化可用于不同类型的样品,但优选用于环境,生物和/或医学样品。

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