Screening group B streptococci.

摘要

A method for detecting the presence or absence of Group B Streptococcus (GBS) in a biological sample from an individual, said method comprising: performing at least one cycling step, wherein a cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of pts primers to produce a pts amplification whether a nucleic acid molecule GBS pts in said sample is present, wherein said hybridizing step comprises contacting said sample with a pair of pts probes, wherein one of the pts probes is labeled with a donor fluorescent moiety and the other pts probe is labeled with a corresponding acceptor fluorescent moiety ; and detecting the presence or absence of energy transfer fluorescence resonance (FRET) between said donor fluorescent moiety and said acceptor fluorescent moiety of said pts probe. (I) wherein the presence or absence of fluorescence is indicative of the presence or absence of GBS in said sample, and (ii) wherein said pair of pts primers comprises a first pts primer and a second pts primer wherein said first pts primer comprises the sequence 5 '- TGA GAA GGC AGT AGA AAG CTT AG-3' (SEQ ID NO: 1), and wherein said second pts primer comprises the sequence 5 '' - TGC ATG TAT GGG TTA TCT TCC-3 '(SEQ ID NO: 2); or wherein said pair of pts probes comprises a first pts probe and a second pts probe, wherein said first pts probe comprises the sequence 5 '- CAA ATT AAA GAG ACT ATT CGT GCA A-3' '( SEQ ID NO: 3), and wherein said second pts probe comprises the sequence 5 '- CAA GTA AAT GCA GAA ACA GG-3' (SEQ ID NO: 4).