THREE-DIMENSIONAL IMAGING OF SINGLE PARTICLES

摘要

A technique of three-dimensional imaging of objects uses an iterative solvent-flattening approach in which a mask is applied to the objects in the spatial domain where detail outside of the mask being discarded, a working image then being transformed into the spatial frequency domain from where the phase information is combined with the original amplitude information to produce a merged updated working image which can then be subject to the same masking and merging steps until appropriate convergence parameters are met. The technique is particularly well suited to imaging single particle objects, such as biological molecules, using electron microscopy.