首页>
外国专利>
LENTIVIRAL VECTORS THAT PROVIDE IMPROVED EXPRESSION AND REDUCED VARIEGATION AFTER TRANSGENESIS
LENTIVIRAL VECTORS THAT PROVIDE IMPROVED EXPRESSION AND REDUCED VARIEGATION AFTER TRANSGENESIS
展开▼
机译:转染后可改善表达并减少变异的细小病毒载体
展开▼
页面导航
摘要
著录项
相似文献
摘要
The present invention provides new lentiviral vectors that include an anti- repressor element (ARE) and, optionally, a scaffold attachment region (SAR). The lentivira vectors provide expression of a heterologous nucleic acid in at least 50% of the cells of multiple cell types when used for lentiviral transgenesis. In certain embodiments of the invention the heterologous nucleic acid encodes an RNAi agent such as an shRNA. The invention further provides transgenic nonhuman animals generated using a lentiviral vector that includes an ARE and optional SAR. In addition, the invention provides a variety of methods for using the vectors including for achieving gene silencing in eukaryotic cells and transgenic animals, and method's of treating disease. The invention also provides animal models ofitiuman disease in which one or more genes is functionally silenced using a lentiviral vector of the invention.
展开▼