METHOD OF CRYSTALLIZING T1 LIPASE FROM GEOBACILLUS SP. STRAIN T1

摘要

A method of crystallizing T1 lipase from Geobacillus sp. strain T1, wherein the method comprises the following steps: (a) Providing purified T1 lipase to be crystallized, (b) Preparing a crystallization mix comprising a buffer comprising 0.1 M NaH2PO4, 0.1 M KH2PO4, 2.0 M NaCl and 0.1 M MES pH 6.5, between 0.5 and 4.5 mg/mL of lipase from step (a), and between 1 and 2 M of precipitant, and (c) Obtaining T1 lipase crystals from step (b) by a hanging-drop methodology, wherein crystals of the lipase are formed between about 1 hour and 1 day. The method is used to generate crystals of the thermostable lipase from Geobacillus stearothermophilus strain T1, with crystallisation occurring at a range of temperatures between 16°C and 70°C.