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A METHOD OF OOCYTE CRYOPRESERVATION INCLUDING PIERCING THE ZONA PELLUCIDA PRIOR TO VITRIFICATION
A METHOD OF OOCYTE CRYOPRESERVATION INCLUDING PIERCING THE ZONA PELLUCIDA PRIOR TO VITRIFICATION
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机译:一种卵囊冷冻保存的方法,包括在玻璃化之前刺破果皮
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摘要
The present invention relates to the cryopreservation and preparation of warmed oocytes for fertilization. Cryopreservation of oocytes using vitrification methods is disclosed. Exemplary techniques include making a vent in the zona pellucida surrounding the oocyte; contacting the oocyte with a vitrification solution comprising at least one cryoprotectant; and vitrifying the oocyte. Further methods of warming the vitrified oocyte, in preparation for intracellular sperm injection are disclosed, allowing for the assessment of successful fertilization.
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