METHOD FOR EXTRACTING FIBRINOGENIC/FIBRINOLYTIC PROTEIN FROM AGKISTRODON BLOMHOFFII USSURIENSIS VENOM

摘要

The invention relates to producing bioactive substances and can be used for biotechnological production processes, in medicine and in the pharmacological industry, in particular to producing medicinal and diagnosis preparations from snake venom. The inventive method consists in carrying affinity chromatography on a column with a Blue Sepharose FF carrier using a buffer solution and in applying it to a column filled with a DEAE Sepharose FF anion exchanger, in washing out unconjugated material from a sorbent by an application buffer, in eluting a fibrinogenic/fibrinolytic protein from the sorbent, in collecting and diluting the protein peak containing said proteins, in applying said peak to a column with a CM Sepharose cation exchanger, in dividing it with a NaCl (0-0.2M) linear gradient in a corresponding buffer, in collecting the protein peak containing the fibrinogenic/fibrinolytic protein and in drying it by lyophilising.