INHIBITION OF P-GLYCOPROTEIN BY TREATMENT WITH A CLASS OF MODULATORS AND THE UIC2 MONOCLONAL ANTIBODY

摘要

The present invention is related to compositions and methods directed at the use of the UIC2 monoclonal antibody with a modulator to combat multidrug resistance. Particularly, the present invention relates to methods, compositions and kits useful for blocking the action of Pgp receptors as well as for detecting cells that exhibit Pgp activity. The embodiments include a method of detecting Pgp-expressing cells using a UIC2 monoclonal antibody, a modulating compound, and a fluorescent substrate. Another embodiment comprises a method of inhibiting the pumping of substrates out of a cell using the UIC2 monoclonal antibody in concert with a modulating compound such as cyclosporine A, vinblastine, valinomycin, PSC 833, o-amsacrine, m-amsacrine or ivermectin.