Nucleic sequences from the genome of Salmonella Typhi, and uses thereof particularly for the in vitro diagnosis of the presence of salmonella bacteria in foodstuffs

摘要

New nucleic acid sequence (I) contains all or part of the genetic information necessary for in vitro infection activity for Salmonella in Hela cultured cells. Also new are (1) any sequence which hybridises with (I) under stringent conditions; (2) sequences (II) of about 200-500 nucleotides from (I) useful as probes, and sequences (III) of 10-30 nucleotides useful as amplification primers; (3) polypeptides (IV) encoded by (I); (4) poly- or mono-clonal antibodies (Ab) recognising (IV) specifically; (5) recombinant nucleic acid (Ia) contg. (I) inserted into a heterologous sequence; and (6) vectors contg. (Ia).