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METHOD FOR ASSAYING CREATINE KINASE ISOZYME ACTIVITY AND ASSAY REAGENT

机译:肌酐激酶同工酶活性测定及试剂测定方法

摘要

A method for measuring the activity of creatine kinase MB (CK-MB) isozyme which is accurate, high in specificity, convenient, by inhibiting the activity of a mitochondria-localized creatine kinase (mCK) isozyme to avoid the influence of mCK and a measurement reagent therefor are provided. A method for measuring the activity of CK-MB isozyme comprising selectively measuring the enzymatic activity of CK isozymes by an immunological inhibition method, wherein after a treatment of selectively and immunologically eliminating the enzymatic actions of CK-M subunit and mCK is performed, the activity of remaining CK is measured.
机译:一种通过抑制线粒体定位的肌酸激酶(mCK)同工酶的活性来避免mCK的影响的一种准确,特异性高,方便的肌酸激酶MB(CK-MB)同工酶的活性的测量方法为此提供了试剂。一种测量CK-MB同工酶活性的方法,其包括通过免疫抑制方法选择性地测量CK同工酶的酶活性,其中在选择性和免疫消除CK-M亚基和mCK的酶促作用的处理之后,进行该活性。测量剩余的CK。

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