METHOD OF DETECTING SPECIFIC BOND REACTION OF MOLECULE BY SINGLE MOLECULE FLUOROMETRY

摘要

It is intended to provide a method of detecting a specific binding of a definite molecule with the use of the single molecule fluorometry technique by which the specific binding of the molecule can be detected and monitored even in the presence of contaminants other than the subject molecule to be detected while avoiding the effects of background light caused by the existence of the contaminants and a non-specific reaction. This method comprises adding, to a mixed sample solution, which consists of a first sample containing a fluorescent labeled first molecule and a second sample containing a second molecule that is to be determined whether or not binding specifically to the first molecule or another second sample that is to be determined whether or not containing a second molecule specifically binding to the first molecule, a bead on which a third molecule specifically binding to the second molecule has been immobilized. When it is determined that the first molecule has been immobilized on the bead based on the fluorescent intensity of the fluorescent label of the first molecule in the mixed sample solution, it is determined that the first molecule has specifically bound to the second molecule or the first sample contains the second molecule.