METHOD OF INDUCING DIFFERENTIATION OF EMBRYONIC STEM CELLS INTO INSULIN-SECRETING CELLS, INSULIN-SECRETING CELLS INDUCED BY THE METHOD AND USE OF THE SAME

摘要

A method comprising differentiating embryonic endoderm, which has been differentiated from undifferentiated embryonic stem cells with the use of activin, into neurogenin 3-expressing cells using a conditioned medium, and then stimulating these cells in a glucose-enriched medium to thereby efficiently induce the differentiation thereof into insulin-secreting cells. In addition to the method of inducing the differentiation of embryonic stem cells into insulin-secreting cells, the insulin-secreting cells induced by this method, a remedy for diabetes, a biological artificial pancreas, a research reagent and a model animal for drug design, each containing the above-described cells, are also provided.