SKIN AND SALIVARY TESTS FOR THE DETECTION OF SENSITIVITY TO OXIDATIVE STRESS BASED THERAPIES

摘要

Radiation therapy (RTx) is standard treatment for prostate cancer (PC). Testosterone increases the basal cellular reactive oxygen species (bROS) levels resulting in a dose-dependent activation of phospho-p38, pAKT and increased expression of clusterin, catalase and manganese superoxide dismutase (MnSOD). These effects are reversible by androgen depletion (AD) or incubation with a reducing agent. Thus, testosterone induces a preset cellular adaptation to radiation through the generation of elevated bROS, which is overturned by AD. The present invention provides a minimally or noninvasive test for determining potential sensitivity to radiation therapy for hormone sensitive tumors; the test comprises measuring the level of basal or stimulated oxidative status of a hormonally regulated tissue and at least one oxidative stress adaptation marker.