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Preconcentration interface coupling capillar electrochromatography to capillary zone electrophoresis
Preconcentration interface coupling capillar electrochromatography to capillary zone electrophoresis
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机译:预浓缩界面将毛细管电色谱耦合到毛细管区带电泳
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摘要
An arrangement for two-dimensional separation of a complex analyte mixture, e.g. of peptides, comprises a first separation device which is a capillary electrochromatography column for the separation in a first dimension and a second device which is a capillary zone electrophoresis channel for the separation in the second dimension. In the area of the interface between the first and the second device a concentration zone is arranged for concentrating the individual sample fractions or components, respectively, leaving the first device before introduction into the second device. The concentration zone is defined in a segment between two or more electrodes seen in flow direction of the sample or sample fractions to be separated. The concentration is achieved by applying a voltage between two electrodes with polarity opposite to the charge of the components of the fraction, sufficiently strong to win the dragging force of the hydrodynamic flow. The introduction into the second device is carried out by turning off the potential difference between the electrodes.
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