METHOD OF PURIFYING ENVIRONMENTAL DNA AND METHOD OF EFFICIENTLY SCREENING PROTEIN-ENCODING GENE FROM ENVIRONMENTAL DNA

摘要

The method of purifying environmental DNA and the method of screening for a gene encoding a functional protein from environmental DNA are provided. DNA from a environmental sample is purified by recovering DNA from an environmental sample and allowing the thus recovered DNA to be subjected to gel filtration chromatography and ion exchange chromatography. In addition, a display library of DNA derived from an environmental sample is created so that screening is carried out in terms of the interaction of the above display library with ligands. Thus, screening of protein-encoding genes can be carried out.